In this tutorial, you will: * Learn how to set up an alternative RNA-Seq pipeline on the T-BioInfo Platform (https://server.t-bio.info/). * Dataset used for pipeline: Humanized mice with implanted breast tumors samples (PDX Project). * Objective: To generate gene expression tables from raw RNA-seq reads (FASTQ files). * Pipeline Workflow: Start → Trimmomatic → PCR Clean → HiSat2 → HTseq → End. Reference Resources Used In Video Tutorial: * Course 5: Transcriptomics - https://learn.omicslogic.com/courses/course/course-5-transcriptomics * Patient Derived Xenograft Models - https://learn.omicslogic.com/courses/course/project-06-patient-derived-xenograft-models * T-BioInfo Platform - https://server.t-bio.info/