In this tutorial, you will: * Learn how to run a differential gene expression analysis at the end of a standard RNA-Seq pipeline on the T-BioInfo Platform. * Dataset used for pipeline: Humanized mice with implanted breast tumors samples (PDX cancer project). * Objective: First generate gene expression tables from the raw RNA-seq reads and then perform differential gene expression analysis with DESEQ2. * Pipeline Workflow: Start → Trimmomatic → PCR Clean → Bowtie2-t → RSemExpTable → DESEQ2 → End. Reference Resources Used In Video Tutorial: * Course 5: Transcriptomics - https://learn.omicslogic.com/courses/course/course-5-transcriptomics * Patient Derived Xenograft Models - https://learn.omicslogic.com/courses/course/project-06-patient-derived-xenograft-models * T-BioInfo Platform - https://server.t-bio.info/