Projects and Tutorials

Differential Expression
Document Video

Differential Gene Expression: Bulk RNA Seq Analysis Results (DESEQ2 Output Review)

User Ratings :

In this tutorial, you will: * Learn about various outputs we get after performing differential gene expression analysis at the end of RNA-Seq pipeline on the T-BioInfo Platform (https://server.t-bio.info/). * Pipeline Workflow: Start → Trimmomatic → PCR Clean → Bowtie2-t → RSemExpTable → DESEQ2 → End. * Outputs obtained range from gene and isoform expression tables to visualization plots tables as well as various interactive visualization graphs such as MultiQC Report, Heatmap, Volcanoplot, etc. * We will also get an external dashboard that allows all the tables and graphs to be maintained in a shareable format. (https://dash.t-bio.info/diffexp?id=3934261-716bdca9bd3b85b7) Reference Resources Used In Video Tutorial: * Course 5: Transcriptomics - https://learn.omicslogic.com/courses/course/course-5-transcriptomics * Patient Derived Xenograft Models - https://learn.omicslogic.com/courses/course/project-06-patient-derived-xenograft-models * T-BioInfo Platform - https://server.t-bio.info/