In this tutorial, you will: * Learn how we can conduct differential gene expression analysis on the T-BioInfo Platform (https://server.t-bio.info/). * Project Dataset used for pipeline: Humanized mice with implanted breast tumors samples (PDX breast cancer project). * Objective: Identify differentially expressed genes and annotate gene sets using Gene Ontology (GO) terms and KEGG pathways in which they are involved. * Pipeline Workflow: Start → DESEQ2 → Enrichment Analysis → GSEA → End. Reference Resources Used In Video Tutorial: * Course 5: Transcriptomics - https://learn.omicslogic.com/courses/course/course-5-transcriptomics * Patient Derived Xenograft Models - https://learn.omicslogic.com/courses/course/project-06-patient-derived-xenograft-models * T-BioInfo Platform - https://server.t-bio.info/